Effects of jnk inhibitor on inflammation and fibrosis in the ovary tissue of a rat model of polycystic ovary syndrome.

نویسندگان

  • Gulay Bulut
  • Zehra Kurdoglu
  • Yeliz Bozdemir Dönmez
  • Mertihan Kurdoglu
  • Remzi Erten
چکیده

OBJECTIVE In our study, we aimed to investigate the effects of Jun N-terminal kinase inhibitor (SP600125) on fibrosis and inflammation in rats with polycystic ovary syndrome (PCOS). METHOD 50 Wistar-albino rats were divided into five groups (n=10 each): control group, sham group, PCOS group, SP600125+ PCOS group and SP600125 group. In the estradiol valerate (EV)-treated group in which PCOS was injected with a single 4 mg/kg i.p. of EV in 0.2 ml sesame oil and the rats were sacrificed on day 60. The estradiol valerate (EV)-treated + SP600125-treated group was injected with a single 4 mg/kg i.p. of EV in 0.2 ml sesame oil. As of day 60, the treatment group was additionally given 15 mg/kg i.p. of SP600125 once daily for 4 consecutive days and the rats were sacrificed on day 65. Histopathological findings (ovarian morphology, edema, inflammatory cell infiltration, vascular congestion and hyperemia) and collagen type IV immunoexpression were assessed. RESULTS The SP600125+ PCOS group showed a significant level of improvement in ovarian follicle morphology, edema, inflammatory infiltrate, vascular congestion and hyperemia as compared with the PCOS group. Furthermore, collagen type IV immunoexpression showed a significant reduction in staining intensity on the theca cell layer and ovary stroma as compared to the PCOS group. CONCLUSION This study demonstrates the therapeutic effect of SP600125 in the prevention of PCOS in an experimental model.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Effect of Sitagliptin on Inflammatory Mediators in the Ovary of Rat with Polycystic Ovary Syndrome

Background and Aims: Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disease in females of reproductive age and is a significant infertility cause. Inflammation plays a crucial role in the pathogenesis of PCOS. The present study evaluated whether sitagliptin, dipeptidyl peptidase-4 inhibitor, attenuates inflammatory markers C-reactive protein (CRP), interleukin(IL)-6, tumor necro...

متن کامل

Survey on the interaction effect of dopamine D2 receptor antagonist on morphine-induced polycystic ovary syndrome in rat

Background and Objective: Polycystic ovary syndrome (PCOS) is one of the most common endocrine and metabolic disorders in premenopausal women. Opioid drugs, including morphine are effective inducers of the PCOS. Hyperprolactinemia also increases the likelihood of this complication. The dopamine, the inhibitor of prolactin secretion, has receptors in the ovarian tissue. In this study, metoclopra...

متن کامل

Hydroalcoholic extract of flaxseed improves polycystic ovary syndrome in a rat model

Objective(s): Herbal medicines are an alternative choice for treatment or controlling of polycystic ovary syndrome (PCOS). Effect of hydroalcoholic extract of flaxseed was evaluated on ovarian hormones and histological changes of uterus and ovary in a PCOS-induced rat model.Materials and Methods: Twenty four rats divided into four groups including negative control, positive control, PCOS and tr...

متن کامل

The Effect of Green Tea Extract on Reproductive Improvement in Estradiol Valerate-Induced Polycystic Ovary Polycystic Ovarian Syndrome in Rat

Polycystic ovary syndrome (PCOS) is a reproductive and metabolic disorder in which thelevel of oxidative elements in blood rises. Green tea is a potent antioxidant since it containscatechins. In this study, the effect of hydro-alcoholic green tea extract on PCOS rats was examined.For PCOS induction, 96 mature Wistar rats were given estradiol valerate. After 60 days, therats were divided into fo...

متن کامل

P-184: Effects of Licorice Hydroalcoholic Root Extract on Letrozole-Induced Polycystic Ovary Syndrome in Mouse

Background: Polycystic ovary syndrome (PCOS) is multifaceted and includes reproductive, metabolic, and general health disorders, so employing different animal models is needed to study the syndrome. Use of an aromatase inhibitor, letrozole, on adult female rats produces a phenotype remarkably similar to that of PCOS. Licorice has been used in treating women infertility for thousands of years. L...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • International journal of clinical and experimental pathology

دوره 8 8  شماره 

صفحات  -

تاریخ انتشار 2015